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KMID : 1094720090140010084
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 1 p.84 ~ p.90
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Amino Acid and Soluble Protein Cocktail from Waste Keratin Hydrolysed by a Fungal Keratinase of Paecilomyces marquandii
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Maria Vesela
Jo?efa Friedrich
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Abstract
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Waste bovine hooves and horns were enzymatically hydrolysed into soluble products intended for foliar fertilizer. With the powdered keratin at 50℃ and pH 8 between 34 to nearly 60% of nitrogen was solubilized in 5 h, depending on the enzyme concentration. The reaction could further be improved by steam pretreatment of the keratin, resulting in 98% solubilisation of the nitrogen. The products of hydrolysis consisted of a mixture of soluble proteins, peptides, and free amino acids. Among the latter, 18 common amino acids were detected. Several of them were previously recognized to have a positive effect on plants. Nonpolar neutral, basic, and sulphur amino acids were present in relatively large amounts, while proline and tryptophan were not found. Comparison with other protein hydrolysates aimed for fertilizer suggests that keratin degradation products, obtained by enzymatic hydrolysis, have potential to be used for foliar fertilization, alone or in a combination with another complementary hydrolysate of a different source, such as skin or plant proteins.
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KEYWORD
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keratinous waste, waste bioconversion, keratin hydrolysis, fungal keratinase, Paecilomyces marquandii, foliar plant fertilizer
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